Monday, January 15, 2007

Revisiting ELISA Spot assays

ELISA spot assays are used for the quantitative determination of single cells secreting a specific cytokine. They are known as ELISA spot assays due to their similarities to the ELISA method, but nowadays, they are more commonly referred to as ELISPOT assays.

Kits are available which contain the basic components and protocols required to perform colorimetric assays wherein one spot marks one cell to be identified.